The
Effects of Vital Cell on Late Middle-Aged and Aged Humans
Materials
and Methods
Cases under observation:
Fifty cases over the age of 55 were chosen, nineteen
of which were in their late middle-ages (55-59) and thirty-one
of which were over the age of 60. Among the elderly, ten cases
were between the ages of 65 and 69, while six of the cases
were over70. The eldest was 77. Of the fifty cases, twenty-three
were male and twenty-seven were female. The average age was
62. No organic diseases existed in any these cases.
Dosage and Method:
Subjects took Vital Cell daily for thirty days as a full course
of treatment.
Observation
Venous blood was drawn from
the fifty cases before and after administration for a lymphocyte
transformation test, an erythrocyte rosette formation test,
and determination of immunoglobulin IgA, IgQ and IgM.
Methods
Lymphocyte
Transformation Test
Reagents: PHA (phytohemagglutinin),
Dongfeng 771 complex culture solution (1g of solution was
added to distilled water and later sterilized and filtered),
inactivated bovine serum, 0.87% NH4Cl solution and 5% NaHCO3
Solution.
Operation: Take a sample
of 2ml of venous blood and place it in a bottle containing
125units of heparin and mix. Take a bottle of culture solution
containing 0.25ml of PHA and add 0.5ml of heparin anticoagulant.
After mixing them, put the bottle into a 37C incubator for
72-hours. Remove all clear fluid from the bottle and add
5ml of 0.87% NH4Cl. Then put the solution back into the
incubator for another twenty minutes. Remove the bottle
and centrifuge the mixture (200,000 r.p.m.). After removing
the clear fluid, put 4-5 drops of the bovine serum into
the sediment and stir. Remove the clear fluid again after
an additional centrifuge treatment (20,000 per five minutes)
and make a smear of the sediment with Wright’s Stain
for microscopic examination.
Erythrocyte
Rosette Formation Test
Principle: Some B-lymphocytes
will, when added to erythrocytes of the mouse, form rosettes
which can serve as a sign of B-cell determination.
Operation: Take the suspension
of mouse erythrocytes, which have been washed tree times
with Hank’s Washing Solution (1% concentration). Then
add the isolated human lymphatic suspension (20 x 10/ml)
in equivalent amounts to the mouse erythrocytes. Mix the
two before incubating the mixture at 37C for thirty minutes.
Centrifuge the mixture sat a low speed for nineteen minutes
and keeps it shaking for an hour at a constant temperature
of 40C. Put a drop of 2.5% glutaraldehyde into the mixture
for a 15-30 minute reaction. Finally, a drop of methylene
blue is added and samples are collected for the count of
rosettes.
Immunoglobulin
Determination
Instrument: BECKMAN (Immunochemical
Analysis System. Produced in the USA).
Reagents: Special reagents produced by
Shanghai Changzheng Hospital were used.
Method: Laser scattering turbidimetry.
- Lymphocyte Transformation Rate:
The pre-administration average was 53.994 + 5.765 and the
post-administration average was 59.444 + 5.305. The T value
was 2.752 and the P value was less than 0.05. They are all
significant.
- Erythrocyte Rosette Formation
Test: The pre-administration average was 52.176
+ 4.172 and the post-administration average was 54.647 +
4.703. The T value was 2.924 and the P value was less than
0.05. They are all significant.
- IgA: The pre-administration
average was 241.378 + 92.865. The P value was more than
0.05.
- IgG: The pre-administration
average was 1460.27 + 319.619 and the post-administration
average was 1454 + 355.386. The P value was more than 0.05.
- IgM: The pre-administration
average was 132.633 and the post-administration average
was 114.309. The P value was more than 0.05.
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